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In order to assess the real impact of ecdysteroids on the course of natural T. gondii proliferation in chronically infected and immunocompromised individuals. Phytoecdysteroids included in the food are most likely not harmful for human or animal health but certain nutrients containing ecdysteroids at high concentrations could promote T. gondii proliferation, demonstrated for the first time in this study, it seems that these compounds should not be taken into consideration as potential medications to treat toxoplasmosis. Judging by the effect of phytoecdysteroids on the T. Additionally, the tested phytoecdysteroids did not stimulate the in vitro secretion of the essential protective cytokines (IFN-γ, IL-2 and IL-10), neither by human nor by murine immune cells involved in an effective intracellular killing of the parasite. Our results showed that phytoecdysteroids promote the multiplication of Toxoplasma in cultures of human or murine immune cells, in contrast to another apicomplexan parasite, Babesia gibsoni. The best-fit IC50 curves were plotted using GraphPad Prism 6.0 (GraphPad Software, Inc.).
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Statistical analysis was performed using SigmaStat 3.5 (Systat Software GmbH). gondii proliferation was measured using radioactivity tests (the level of 3 uracil incorporation by toxoplasms or 3 thymidine by PBMCs and splenocytes), which was confirmed by quantitative Real-Time PCR.
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The influence of phytoecdysteroids on PBMCs, splenocytes and T. The effects of phytoecdysteroids on the viability of human PBMC and mouse splenocytes were evaluated using the MTT assay. gondii DX strain were also used in this study as a source of splenocytes. Non-infected mice (C57BL/6, N = 10 and BALB/c, N = 14) and mice (BALB/c, N = 10) challenged intraperitoneally with 5 tissue cysts of the T. Peripheral mononuclear blood cells were isolated from Toxoplasma-positive and Toxoplasma-negative women (N = 43) and men (N = 21). gondii growth in phytoecdysteroid-treated immunocompetent cells of selected hosts: humans and two strains of inbred mice with genetically determined different susceptibility to toxoplasmosis. This objective was accomplished by the in vitro evaluation of T. for new effective drugs against human and animal toxoplasmosis we decided to test the anti-Toxoplasma potential of phytoecdysteroids (α-ecdysone and 20-hydroxyecdysone) characterized by the pleiotropic activity on mammalian organisms including the enhancement of host's anti-parasitic defence.
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7 Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland. 6 Department of Microbial Genetics, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland. 5 Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland. 4 Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland. 3 Institute of Medical Biology of the Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland. 2 Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland.
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